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Glucose‐induced oscillations of intracellular Ca 2+ concentration resembling bursting electrical activity in single mouse islets of Langerhans
Author(s) -
Valdeolmillos Miguel,
Santos Rosa M.,
Contreras Diego,
Soria Bernat,
Rosario Luis M.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81484-x
Subject(s) - bursting , islet , intracellular , biophysics , chemistry , endocrinology , medicine , biology , insulin , biochemistry , neuroscience
Intracellular Ca 2+ levels were monitored in single, acutely isolated mouse islets of Langerhans by dual emission Indo‐1 fluorometry. High‐frequency (3.1 min −1 ) [Ca 2+ ] i , oscillations with a brief rising time (1–2 s) and 10 s half‐width (‘fast’ oscillations) were detected in 11 mM glucose. Raising the glucose concentration to 16.7 mM increased the duration of these oscillations, which were otherwise absent in 5.5 mM glucose. [Ca 2+ ] i waves of lower frequency (0.5 min −1 ) and longer rising time (‘slow’ oscillations) were also recorded. The data indicate that “fast” oscillations are directly related to β‐cell bursting electrical activity, and suggest the existence of extensive networks of electrically coupled cells in the islet.