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Evidence for reversible multiple redox transformations of [3Fe‐4S] clusters
Author(s) -
Armstrong Fraser A.,
Butt Julea N.,
George Simon J.,
Hatchikian E.Claude,
Thomson Andrew J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81483-8
Subject(s) - redox , cyclic voltammetry , electron transfer , desulfovibrio , ferredoxin , chemistry , cluster (spacecraft) , pyrolytic carbon , crystallography , electrochemistry , voltammetry , inorganic chemistry , electrode , photochemistry , biochemistry , sulfate , organic chemistry , pyrolysis , computer science , programming language , enzyme
Analysis of the diffusionless cyclic voltammetry of ferredoxin III from Desulfovibrio africanus , co‐adsorbed with neomycin as an electroactive film on pyrolytic graphite ‘edge’ electrodes, shows that the reduced [3Fe‐4S] 0 cluster undergoes further fast, chemically reversible, two ‐electron reduction, at a potential E 0 ' = ca. −720 mV (pH 7.15, 0°C). The pH dependence of E 0 ' (pH 6.25–7.80) indicates net transfer of two H + . Observation of similar voltammetric waves in other proteins specifically containing [3Fe‐4S] centres suggests that extensive redox activity and stabilisation of what is formally an all‐Fe(II) species, may be a common, perhaps characteristic, feature of this cluster type.