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In vitro translation of halobacterial mRNA
Author(s) -
Gropp R.,
Oesterhelt D.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81481-4
Subject(s) - ribosome , messenger rna , translation (biology) , in vitro , protein biosynthesis , transfer rna , chemistry , biochemistry , nuclease , rna , biology , microbiology and biotechnology , gene
A system for in vitro translation of halobacterial mRNA is described. It is composed of 70S ribosomes, a nuclease‐treated S100 supernatant, yeast tRNA and halobacterial mRNA. Specific expression of proteins could be demonstrated by translation of messages from halophage ϑ1‐infected cells. However, under no conditions could a pure bacterio‐opsin message be observed to be active in translation. This points to a more complex translational property of bacterio‐opsin and possibly other membrane proteins in archaebacteria.