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Influence of non‐esterified fatty acids on respiratory control of reconstituted cytochrome‐ c oxidase
Author(s) -
Thiel Carsten,
Kadenbach Bernhard
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81469-3
Subject(s) - cytochrome c oxidase , chemistry , biochemistry , respiratory system , enzyme , medicine
Bovine heart cytochrome‐ c oxidase was reconstituted in liposomes (asolectin) and the activity measured in the presence and absence of uncoupler at increasing concentrations of non‐esterified fatty acids. Palmitic and stearic acids resulted in a decrease of about 40% in the respiratory control ratio at a concentration of 1 νM, when measured using a spectrophotometric procedure but not with a polarographic assay method. At higher fatty acid concentrations no further change was found. A 50% decrease in respiratory control was determined when the enzyme was reconstituted in pure phosphatidylcholine containing 2% cardiolipin. The respiratory control of reconstituted cytochrome‐ c oxidase from bovine liver was not influenced by fatty acids.