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Synthesis of human endothelin‐1 precursors in Escherichia coli
Author(s) -
Watanabe Takuya,
Itoh Yasuaki,
Ogi Kazuhiro,
Kimura Chiharu,
Suzuki Nobuhiro,
Onda Haruo
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81466-8
Subject(s) - escherichia coli , complementary dna , endothelin receptor , western blot , microbiology and biotechnology , chemistry , endothelin 1 , northern blot , immunoassay , biochemistry , biology , gene , antibody , immunology , receptor
Endothelin, the most potent vasoconstrictor found in nature, is thought to be important in the regulation of blood pressure and/or local blood distribution. Human placenta cDNA fragment encoding preproendothelin‐1 (preproET‐ 1) and its carboxyl terminal mature precursor (C‐matured precursor) was expressed in E. coli . These products were characterized by both enzyme immunoassay and Western blot analysis.