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Reductive dehydroxylation of 4‐hydroxybenzoyl‐CoA to benzoyl‐CoA in a denitrifying, phenol‐degrading Pseudomonas species
Author(s) -
Glöckler Rainer,
Tschech Andreas,
Fuchs Georg
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81461-9
Subject(s) - denitrifying bacteria , pseudomonas , phenol , chemistry , food science , organic chemistry , denitrification , bacteria , biology , nitrogen , genetics
The initial reactions in anaerobic degradation of phenol to CO 2 have been studied in vitro with a denitrifying Pseudomonas strain grown with phenol and nitrate in the absence of molecular oxygen. Phenol has been proposed to be carboxylated to 4‐hydroxybenzoate [(1987) Arch. Microbiol. 148, 213–217]. 4‐Hydroxybenzoate was activated to 4‐hydroxybenzoyl‐CoA by a coenzyme A ligase. Cell extracts also catalyzed the reductive dehydroxylation of 4‐hydroxybenzoyl‐CoA to benzoyl‐CoA with reduced benzyl viologen as electron donor. This enzyme, benzoyl‐CoA:(acceptor) 4‐oxidoreductase (hydroxylating) (EC 1.3.99.‐), has not been reported before. The data suggest that phenol and 4‐hydroxybenzoate are anaerobically metabolized by this strain via benzoyl‐CoA.