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The cDNA of the two isoforms of bovine cGMP‐dependent protein kinase
Author(s) -
Wernet W.,
Flockerzi V.,
Hofmann F.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81453-x
Subject(s) - complementary dna , microbiology and biotechnology , edman degradation , gene isoform , peptide sequence , biology , cdna library , messenger rna , protein kinase a , biochemistry , northern blot , protein primary structure , kinase , gene
cDNAs encoding the isoform Iα of the cGMP‐dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in λgt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207–4218]. Alternate cDNA clones were isolated which code for a protein slightly different within the aminoterminal part from the known amino acid sequence. These alternate cDNAs contain the sequence of a peptide identified in the isoform 1β of cGMP‐dependent protein kinase. Northern blot analysis of poly(A) + RNA from bovine trachea smooth muscle indicated the presence of two different mRNA species of about 6.2 kb.