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In vitro transcription of the mouse whey acidic protein promoter is affected by upstream sequences
Author(s) -
Lubon Henryk,
Pittius Christoph W.,
Hennighausen Lothar
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81449-8
Subject(s) - transcription (linguistics) , in vitro , promoter , nucleic acid , microbiology and biotechnology , transcription factor , gene , biology , upstream activating sequence , chemistry , biochemistry , gene expression , philosophy , linguistics
The promoter sequences from −175 to +10 of the mouse whey acidic protein (WAP) gene are a target for sequence‐specific binding of nuclear proteins [(1987) Nucleic Acids Res. 15, 2103–2121]. Using in vitro transcription assays based on nuclear extracts, transcription factors were shown to bind to these sequences and promoter upstream sequences were found to stimulate transcription.