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‘Perforated patch recording’ allows long‐term monitoring of metabolite‐induced electrical activity and voltage‐dependent Ca 2+ currents in pancreatic islet B cells
Author(s) -
Falke Lee C.,
Gillis Kevin D.,
Pressel David M.,
Misler Stanley
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81448-6
Subject(s) - patch clamp , islet , metabolite , electrophysiology , chemistry , neurotransmitter , endocrinology , biophysics , medicine , biology , central nervous system , insulin
We describe the application of ‘perforated patch recording’ using the pore‐forming antibiotic nystatin, to monitor the electrical activity and underlying ionic currents of rat and human pancreatic islet B cells. We demonstrate that glucose‐induced electrical activity is seen even in single B cells during current‐clamp recordings lasting hours ‘ L ‐type’ Ca 2+ ‐channel currents can also be monitored over this period of time. This technique may prove useful in examining hormone and neurotransmitter modulation of electrical activity in B cells, while minimizing the effects of cytoplasmic ‘wash‐out’.