Premium
Control of fructose 2,6‐bisphosphate levels in rat macrophages by glucose and phorbol ester
Author(s) -
Bustos R.,
Sobrino F.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81444-9
Subject(s) - phorbol ester , fructose , fructose 2,6 bisphosphate , chemistry , biochemistry , phorbol , protein kinase c , glycolysis , metabolism , enzyme , phosphofructokinase
The presence of fructose 2,6‐bisphosphate (Fru 2,6‐P 2 ) in elicited peritoneal macrophages of rat was examined. These cells possess an active phosphofructokinase‐2 which is diminished by citrate and only slightly inhibited by glycerol 3‐phosphate. Phosphofructokinase‐1 submaximal activity was increased 26‐fold by the addition of 1 μM Fru 2,6‐P 2 . Incubation of cells without glucose decreased the amount of Fru 2,6‐P 2 to zero, but further addition of 5 mM glucose increased the levels of the sugar ester 20‐fold. In addition, the presence of phorbol ester potentiated the synthesis of Fru 2,6‐P 2 . By contrast phenylisopropyladenosine or prostaglandin F 2α inhibited the production of Fru 2,6‐P 2 .