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Effects of pretreatment with tetradecanoyl phorbol acetate on regulation of growth hormone and prolactin secretion from ovine anterior pituitary cells
Author(s) -
Boyd Robert S.,
Wallis Michael
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81436-x
Subject(s) - prolactin , medicine , endocrinology , somatostatin , anterior pituitary , secretion , protein kinase c , chemistry , phorbol , protein kinase a , hormone , prolactin cell , receptor , pituitary gland , kinase , biology , biochemistry
Tetradecanoyl phorbol acetate (TPA) stimulates growth hormone (GH) and prolactin secretion from ovine anterior pituitary cells. Pretreatment of the cells with TPA abolishes this effect, presumably due to down‐regulation of protein kinase C. Such pretreatment did not alter effects of thyrotropin‐releasing hormone or dopamine on prolaction secretion, suggesting no involvement of protein kinase C. Pretreatment with TPA attenuated actions of GH‐releasing hormone on GH release (but not actions on cyclic AMP levels) possibly due to depletion of cellular stores of GH. Such pretreatment also attenuated inhibition of GH release by somatostatin, possibly due to phosphorylation of receptors or associated proteins by protein kinase C.