Premium
Substrate structural requirements of Schizosaccharomyces pombe RNase P
Author(s) -
Drainas Denis,
Zimmerly Steven,
Willis Ian,
Söll Dieter
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81433-4
Subject(s) - schizosaccharomyces pombe , rnase p , transfer rna , rnase ph , schizosaccharomyces , rnase mrp , chemistry , mutant , substrate (aquarium) , biochemistry , gene , stereochemistry , biology , rna , ecology
RNase P from Schizosaccharomyces pombe has been purified over 2000‐fold. The apparent K m for two S. pombe tRNA precursors derived from the supS1 and sup3‐e tRNA Ser genes is 20 nM; the apparent V max is 2.5 nM/min ( supS1 ) and 1.1 nM/min ( sup3‐e ). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.