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Effect of phenobarbital on the expression of glutathione S ‐transferase isoenzymes in cultured rat hepatocytes
Author(s) -
Vandenberghe Yves,
Morel Fabrice,
Foriers André,
Ketterer Brian,
Vercruysse Antoine,
Guillouzo André,
Rogiers Vera
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81428-0
Subject(s) - phenobarbital , isozyme , glutathione s transferase , glutathione transferase , glutathione , chemistry , hepatocyte , biochemistry , microbiology and biotechnology , enzyme , biology , endocrinology , in vitro
Cultured adult rat hepatocytes were treated daily with 3.2 mM phenobarbital (PB) in order to study its effect on the expression of cytosolic glutathione S ‐transferase isoenzymes. Glutathione S ‐transferase (GST) activities, using 1‐chloro‐2,4‐dinitrobenzene and 1,2‐dichloro‐4‐nitrobenzene as substrates, were increased when PB was present in the culture medium. After purification and separation of GST on glutathione Sepharose 6 B and reversed‐phase HPLC, respectively, it was observed in vitro that PB caused an increase in the relative amounts of subunits 1, 3 and 7 compared to subunits 2 and 4. Using Northern blot technique, elevated levels of GST subunit 1/2 and 7 mRNA were measured, after addition of PB to the cultures.