Premium
Adenine nucleotides regulate Ca 2+ transport in brain mitochondria
Author(s) -
Rottenberg Hagai,
Marbach Miriam
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81396-1
Subject(s) - mitochondrion , spermine , adenine nucleotide translocator , chemistry , efflux , adenine nucleotide , biophysics , biochemistry , nucleotide , biology , enzyme , gene
Adenine nucleotides (ADP>ATP) greatly enhance Ca 2+ uptake and retention in rat brain mitochondria. In the presence of both spermine and ADP, brain mitochondria sequester Ca 2+ down to cellular free Ca 2+ levels, suggesting a role for mitochondria in modulating Ca 2+ cycles in brain cells. Analysis of the effects of various inhibitors on Ca 2+ uptake and efflux suggest that locking the ADP/ATP translocator in its M‐state stimulates electrogenic Ca 2+ uptake and, to a lesser extent, inhibits Ca 2+ efflux. It is suggested that this effect is due to a modulation of the surface charge on the M‐side which enhances Ca 2+ dissociation from the carriers.