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Zinc increases phorbol ester receptors in intact B‐cells, neutrophil polymorphs and platelets
Author(s) -
Forbes I.J.,
Zalewski P.D.,
Hurst N.P.,
Giannakis C.,
Whitehouse M.W.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81388-2
Subject(s) - protein kinase c , receptor , cytosol , platelet , phorbol ester , phorbol , chemistry , ionophore , zinc , biochemistry , cell fractionation , microbiology and biotechnology , biology , kinase , enzyme , membrane , immunology , organic chemistry
In the presence of pyrithione, which was used as a Zn 2+ ionophore, Zn 2+ (10–100 μM) increased phorbol ester binding by intact B‐CLL cells in a dose‐dependent fashion. Zn pyrithione increased 2‐fold the number of phorbol ester receptors in B‐cells (0.74 to 1.4 pmol/10 6 cells), neutrophil polymorphs (0.2 to 0.51 pmol/10 6 cells) and platelets (91 to 209 pmol/10 10 cells). Fractionation of cells after treatment with Zn pyrithione showed that increased binding of PDBu occurred in the particulate fraction of cells and this was accompanied by loss of phorbol ester receptors from the cytosol. These data are compatible with a role for Zn in the subcellular distribution and activation of protein kinase C.