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Spatial localization of the stimulus‐induced rise in cytosolic Ca 2+ in bovine adrenal chromaffin cells
Author(s) -
Cheek Timothy R.,
O'Sullivan Antony J.,
Moreton Roger B.,
Berridge Michael J.,
Burgoyne Robert D.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81385-7
Subject(s) - cytosol , chromaffin cell , stimulus (psychology) , biophysics , chemistry , endocrinology , microbiology and biotechnology , adrenal medulla , neuroscience , medicine , biology , biochemistry , psychology , enzyme , catecholamine , psychotherapist
The spatial distribution of the intracellular free Ca 2+ (Ca 2+ i ) rise elicited by different stimuli in bovine adrenal chromaffin cells was examined in single fura‐2‐loaded cells. In response to the potent secretagogues nicotine and high K + , Ca 2+ i was initially localized exclusively to the entire subplasmalemmal area of the cell. In response to the ineffective secretagogues, methacholine and muscarine, the rise in Ca 2+ i originated only in one pole of the cell and even at the peak of the response Ca 2+ was still generally restricted to this same area of the cell. These results suggest that the triggering of exocytosis from these cells requires a specific spatial distribution of Ca 2+ i .