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Galanin inhibits insulin secretion by direct interference with exocytosis
Author(s) -
Ullrich Susanne,
Wollheim Claes B.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81379-1
Subject(s) - galanin , exocytosis , pertussis toxin , gtp' , secretion , medicine , endocrinology , neuropeptide , g protein , insulin , second messenger system , chemistry , biology , microbiology and biotechnology , signal transduction , biochemistry , receptor , enzyme
Electrically permeabilized RINm5F cells were used to study whether galanin inhibits insulin secretion distally to the generation of soluble second messengers. Ca 2+ ‐induced insulin secretion was inhibited by the neuropeptide in a dose‐dependent manner. Galanin appears to act via a G‐protein as persussis toxin treatment abolished the effect. GTP (100 μM), GDP (100 μM) and a low dose of GTPγS (10 μM) did not affect galanin‐mediated inhibition of secretion. In contrast, at 100 μM, GTPγS attenuated and GDPβS abolished the effect of the peptide. We conclude that galanin inhibits exocytosis directly by a mechanism involving a G‐protein.