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Inhibition of HIV replication in cell culture by the specific aspartic protease inhibitor pepstatin A
Author(s) -
von der Helm Klaus,
Gürtler Lutz,
Eberle Josef,
Deinhardt Friedrich
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81368-7
Subject(s) - pepstatin , reverse transcriptase , incubation , cell culture , virology , protease , protease inhibitor (pharmacology) , biology , human immunodeficiency virus (hiv) , enzyme , biochemistry , rna , viral load , antiretroviral therapy , gene , genetics
After incubation of H9 cells infected with human immunodeficiency virus (HIV) with pepstatin A at 10 −4 M for 2, 4, or 11 days, the culture medium contained significantly less HIV core antigen (p24) than controls without pepstatin A and no or only borderline activity of reverse transcriptase was detected. In addition, after pepstatin A treatment no infectious HIV at 2 or 4 days and only minimal amounts at 11 days were detectable in the culture medium.