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The yeast pyruvate kinase gene does not contain a string of non‐ preferred codons: Revised nucleotide sequence
Author(s) -
McNally Teresa,
Purvis Ian J.,
Fothergill-Gilmore Linda A.,
Brown Alistair J.P.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81359-6
Subject(s) - sequence (biology) , gene , nucleic acid sequence , biochemistry , yeast , pyruvate kinase , chemistry , nucleotide , genetics , biology , enzyme , glycolysis
The sequence of the gene encoding pyruvate kinase from Saccharomyces cerevisiae was re‐determined because of failures with oligonucleotide‐directed mutagenesis experiments involving a region thought to contain a string of five contiguous non‐preferred codons. This region was found to be difficult to sequence and was shown to have three extra bases when compared with the published sequence [(1983) J. Biol. Chem. 258, 2193–2201]. The revised sequence demonstrates that the yeast pyruvate kinase gene does not have a cluster of non‐preferred codons, and that it therefore is not an example of the class of genes which possibly exhibit translational control by the presence of non‐preferred codons.