Premium
Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells
Author(s) -
Hoosein Naseema M.,
Thim Lars,
Jørgensen Klavs H.,
Brattain Michael G.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81357-2
Subject(s) - pancreatic polypeptide , thymidine , endocrinology , medicine , cell growth , cell culture , estrogen , chemistry , microbiology and biotechnology , biology , cancer research , hormone , biochemistry , in vitro , glucagon , genetics
The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [ 3 H]thymidine into HCT 116 cells as well as cell proliferation in a dose‐dependent manner. Maximal increase in [ 3 H]thymidine incorporation of 50–60% occurred at 3–300, μM PSP. The VIP‐mediated‐increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen‐induced pS2 protein in MCF‐7 breast cancer cells. We find that PSP also enhanced [ 3 H]thymidine incorporation in MCF‐7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.