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Regulation of LacZ mRNA translatability in a cell‐free system at heat shock by the last four sense codons
Author(s) -
Denisenko Oleg N.,
Yarchuk Oleg B.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81346-8
Subject(s) - reticulocyte , xenopus , messenger rna , translation (biology) , sense (electronics) , microbiology and biotechnology , biology , hsp70 , cell free system , lac operon , heat shock protein , gene , gene expression , chemistry , genetics , in vitro
Under heat shock conditions translation of Xenopus laevis normal mRNAs in a rabbit reticulocyte cell‐free system is blocked whereas hsp70 mRNA is translated. mRNA for E. coli β‐galactosidase containing the last four sense codons of Drosophila hsp70 at its 3′‐end was constructed. This mRNA is efficiently translated in a rabbit reticulocyte cell‐free system at 43°C.