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Defect in phorbol acetate‐induced translocation of diacylglycerol kinase in erbB‐transformed fibroblast cells
Author(s) -
Kato Miwako,
Kawai Sadaaki,
Takenawa Tadaomi
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81345-6
Subject(s) - phosphatidylinositol , diacylglycerol kinase , chromosomal translocation , cytosol , protein kinase c , fibroblast , kinase , microbiology and biotechnology , chemistry , biology , biochemistry , in vitro , enzyme , gene
We here show that tetradecanoyl phorbol acetate (TPA) and 1‐oleoyl 2‐acetyl glycerol (OAG) cause the translocation of diacylglycerol (DG) kinase from the cytosol to the membrane fractions in chick embryo fibroblast (CEF) cells. However, this translocation is not marked in erbB‐transformed chick embryo fibroblast (GEV) cells. The activities of phosphatidylinositol (PI) and phosphatidylinositol 4‐phosphate (PIP) kinases in membrane fractions are not altered by TPA treatment in either CEF or GEV cells. Such reduced translocation of DG kinase by TPA is also observed in src‐transformed cells, but not in myc‐transformed cells. These results suggest that the defect in DG kinase translocation may result in failure to suppress the overactivation of protein kinase C in erbB‐2 and src‐transformed cells, which may lead to cell growth and transformation.