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Leucine transport in plasma membrane vesicles of Saccharomyces cerevisiae
Author(s) -
Calahorra M.,
Opekarová M.,
Ramirez J.,
Peña A.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81342-0
Subject(s) - saccharomyces cerevisiae , vesicle , chemistry , biochemistry , membrane , membrane transport , leucine , biophysics , microbiology and biotechnology , yeast , biology , amino acid
Yeast plasma membrane vesicles were obtained by the fusion of liposomes with purified yeast membranes by means of the freeze thaw‐sonication technique. Beef heart mitochondria cytochrome‐ c oxidase was incorporated into the vesicles. Addition of substrate (ascorbate/TMPD/cytochrome c ) generated a membrane potential negative inside, and an alkaline pH gradient inside the vesicle, that served as the driving force for leucine transport. Both ΔpH and ΔΨ could drive leucine transport. When ΔpH was increased in the presence of valinomycin and potassium, at the expense of ΔΨ, leucine uptake increased by 10%.