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Stimulation of polyphosphoinositide hydrolysis in Swiss 3T3 cells by recombinant fibroblast growth factors
Author(s) -
Brown Kenneth D.,
Blakeley Diana M.,
Brigstock David R.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81340-7
Subject(s) - stimulation , 3t3 cells , bombesin , fibroblast growth factor , inositol phosphate , inositol , recombinant dna , chemistry , endocrinology , medicine , fibroblast , biochemistry , biology , in vitro , receptor , transfection , neuropeptide , gene
Mitogenic concentrations of recombinant acidic or basic fibroblast growth factor (FGF) stimulated the accumulation of [ 3 H]inositol phosphates ([ 3 H]IPs) in Swiss 3T3 cells pre‐labelled for 48 h with [ 3 H]inositol. Maximal effects were obtained at 0.3 ng/ml and 3 ng/ml for basic and acidic FGF, respectively. Higher doses of either factor led to a diminished stimulation. FGF also stimulated 45 Ca 2+ release from cells pre‐labelled with the isotope. However, FGF‐stimulated production of [ 3 H]IPs and release of 45 Ca 2+ exhibited marked differences when compared with the responses to the peptide mitogen bombesin; the FGF responses were markedly slower and were not inhibited by phorbol esters.