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Production of antibodies against the coenzyme pyrrolequinoline quinone
Author(s) -
Citro G.,
Verdina A.,
Galati R.,
Floris G.,
Sabatini S.,
Finazzi-Agrò A.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81334-1
Subject(s) - quinone , polyclonal antibodies , chemistry , biochemistry , antibody , amine oxidase , cofactor , western blot , enzyme , biology , immunology , gene
Polyclonal antibodies against pyrrolequinoline quinone have been elicited in rabbits. These antibodies react with free and protein‐bound pyrrolequinoline quinone. In particular they react with native and denatured lentil seedling amine oxidase as detected by dot‐blot and ELISA assays. The presence of 1 mol pyrrolequinoline quinone per mol of enzyme was determined by the last method.