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Na + ‐independent Mg 2+ efflux from Mg 2+ ‐loaded human erythrocytes
Author(s) -
Günther T.,
Vormann J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81329-8
Subject(s) - efflux , bumetanide , chemistry , extracellular , symporter , cotransporter , dids , amiloride , biophysics , ion transporter , biochemistry , sodium , membrane , transporter , biology , organic chemistry , gene
Net Mg 2+ efflux from Mg 2+ ‐loaded human erythrocytes was maximal after reincubation in sucrose. Net Mg 2+ efflux was not inhibited by furosemide or bumetanide and, therefore, was not performed by the (Na,K,Cl)‐ or (K,Cl)‐cotransport system. A component of net Mg 2+ efflux was inhibited by extracellular NaCl, KCl, LiCl, choline Cl and SITS, in analogy to the inhibition of net Cl − and SITS. Therefore, it was concluded that net Mg 2+ efflux is dependent on net Cl − efflux for charge compensation. Cl − ‐dependent net Mg 2+ efflux was inhibited by amiloride. Only 10% of the maximal net Mg 2+ efflux may depend on extracellular Na + .