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The prostate‐specific antigen gene and the human glandular kallikrein‐1 gene are tandemly located on chromosome 19
Author(s) -
Riegman P.H.J.,
Vlietstra R.J.,
Klaassen P.,
van der Korput J.A.G.M.,
van Kessel A.Geurts,
Romijn J.C.,
Trapman J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81253-0
Subject(s) - kallikrein , microbiology and biotechnology , biology , gene , gene cluster , complementary dna , prostate specific antigen , antigen , pancreas , gene family , cdna library , southern blot , genetics , prostate , gene expression , endocrinology , biochemistry , cancer , enzyme
Using a prostate‐specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate‐specific antigen, human glandular kallikrein‐1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate‐specific antigen gene and the human glandular kallikrein‐1 gene overlap and span a region of about 36 kb. The two genes are aligned in a head to tail orientation at a mutual distance of 12 kb. Southern blot analysis of DNA from a panel of human‐hamster hybrid cells with specific probes revealed the genes to be situated on chromosome 19. Assuming that the pancreas/kidney kallikrein gene is located in the same cluster, the distance to the prostate‐specific antigen gene and the human glandular kallikrein gene must be at least 15 kb.