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Monitoring the transport and phosphorylation of 2‐deoxy‐D‐glucose in tumor cells in vivo and in vitro by 13 C nuclear magnetic resonance spectroscopy
Author(s) -
Navon Gil,
Lyon Robbe C.,
Kaplan Ofer,
Cohen Jack S.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81246-3
Subject(s) - in vivo , in vitro , nuclear magnetic resonance spectroscopy , phosphorylation , chemistry , nuclear magnetic resonance , cancer research , biochemistry , biology , stereochemistry , physics , genetics
We describe the use of 2‐deoxy‐D‐[6‐ 13 C]glucose to follow simultaneously, by 13 C NMR, both transport and phosporylation to its 6‐phosphate form, in MCF‐7 breast cancer cells in vitro and in vivo in subcutaneous tumors in nude mice.