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Two forms of the GABA A receptor distinguished by anion‐exchange chromatography
Author(s) -
Moffett John R.,
Namboodiri Aryan,
Neale Joseph H.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81245-1
Subject(s) - chemistry , flunitrazepam , gabaa receptor , muscimol , chromatography , high performance liquid chromatography , elution , ion chromatography , receptor , affinity chromatography , resolution (logic) , membrane , biochemistry , enzyme , computer science , artificial intelligence
The GABA A receptor complex was solubilized from rat brain membranes in Triton X‐100, enriched by 1012‐S affinity chromatography, and subjected to DEAE anion‐exchange chromatography. Two forms were distinguished by their differential elution during this HPLC with a KCl gradient. They displayed similar [ 3 H]muscimol‐ and [ 3 H]flunitrazepam‐binding characteristics, as well as [ 3 H]flunitrazepam‐binding inhibition by CL 218872. Rechromatography of these distinct ionic forms indicated that they were not in dynamic equilibrium during chromatography. Resolution of these two pharmacologically similar populations of GABA A receptor by anion‐exchange HPLC suggests that they differ in charge densities, a condition which may reflect differing glycosylation or phosporylation states of the complex.