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The developmental regulation of the L2/HNK‐1 and L3 carbohydrate epitopes in mouse brain Evidence for separate control of lipid‐ and protein‐bound epitopes
Author(s) -
Breen Kieran C.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81235-9
Subject(s) - epitope , carbohydrate , chemistry , biology , glycoprotein , biochemistry , microbiology and biotechnology , antibody , immunology
The carbohydrate epitopes L2/HNK‐1 and L3 have previously been identified on various neural cell adhesion molecules and have been suggested to play a role in the mediation of cell‐cell adhesion. In this study, the developmental expression of the two epitopes in soluble, membrane‐bound and chloroform/methanol‐extracted fractions of the constituent mouse brain regions was examined by enzyme‐linked immunosorbent assay (ELISA). The protein‐bound epitopes were shown to be uniformly developmentally regulated, with levels peaking at postnatal day 20 (P20). The epitopes in a crude chloroform/methanol fraction, however, demonstrated a different pattern, with L2 peaking earlier at postnatal day zero (P0). These results suggest a possible interaction between the control of the two pools of the epitope.