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Growth stimulation by bombesin does not involve activation of Na + /H + exchange in chick embryo otic vesicles in vitro
Author(s) -
Gillespie J.I.,
Giraldez F.,
Greenwell J.R.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81208-6
Subject(s) - bombesin , vesicle , stimulation , in vitro , biology , embryo , cell growth , microbiology and biotechnology , medicine , chemistry , endocrinology , biochemistry , neuropeptide , receptor , membrane
The effect of the mitogen bombesin on pH i in cells of the chick otic vesicle was studied using dual wavelength microspectrofluorimetric techniques. The results show that the bombesin did not produce an immediate alkalinization in quiescent otic vesicle cells nor a long‐term change in pH i in vesicles reactivated to grow in culture. The recovery of pH i from an acid load, caused by an NH 4 pulse, involved both Na + /H + exchange and Na + :HCO 3 influx mechanisms, neither of which was influenced by bombesin. These observations do not fit with a general model whereby pH i is a universal signal for DNA replication and cell proliferation.