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Synthesis of paf‐acether from exogenous precursors by the prokaryote Escherichia coli
Author(s) -
Denizot Yves,
Dassa Elie,
Kim Hee-Yong,
Bossant Marie-Jeanne,
Salem Norman,
Thomas Yolène,
Benveniste Jacques
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81207-4
Subject(s) - prokaryote , escherichia coli , bacteria , mediator , chemistry , biological activity , platelet activating factor , microbiology and biotechnology , biochemistry , biology , in vitro , immunology , gene , genetics
Paf‐acether (paf) is a potent mediator of inflammatory diseases and septic shock. Using normal‐phase HPLC, a paf‐like activity was found in culture supernatants from E. coli . Prokaryotic paf exhibited the same biological and physico‐chemical properties as eukaryotic cells and synthetic paf. Further, reverse‐phase HPLC indicates that paf generated by bacteria is predominantly of the hexadecyl and octadecyl species. When cultures were supplemented with lyso‐paf, a dramatic increase in paf production was observed. The purity and molecular structure of bacterial paf were further characterized by mass spectral analysis. These results could be of importance considering the pathogenetic role of enterobacteria. Further, it appears that the competence to form and release paf is an early phylogenetic development.