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Effects of solute conditions on the relative affinities of the oligonucleotides d(G‐C) 5 and d(A‐T) 5 for the anthracycline drug 2‐fluoro‐4‐demethoxydaunomycin
Author(s) -
Hammer Brendon C.,
Russell Richard A.,
Warrener Ronald N.,
Collins J.Grant
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81198-6
Subject(s) - affinities , lysine , arginine , chemistry , aqueous solution , anthracycline , stereochemistry , amino acid , biochemistry , biology , organic chemistry , cancer , breast cancer , genetics
19 F NMR has been used to show that changes in NaCl concentration, as well as the presence of lysine or arginine, affect the equilibrium distribution of the synthetic anthracycline 2‐fluoro‐4‐demethoxydaunomycin (2FD) between binding sites on d(G‐C) 5 and d(A‐T) 5 in a 1:1:1 molar aqueous system: 2FD/d(G‐C) 5 /d(A‐T) 5 . Varying the pH between 6.2 and 7.7 had no effect. NaCl concentrations below 0.1 M led to a d(G‐C) 5 preference while above 0.1 M a preference for d(A‐T) 5 was observed. At comparable solute concentrations, use of either lysine or arginine resulted in a significant drug preference for d(G‐C) 5 compared to systems containing only NaCl.