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Phosphorylation of NADPH‐cytochrome c reductase in guinea pig peritoneal macrophages stimulated with phorbol myristate acetate
Author(s) -
Tamoto Koichi,
Hazeki Kaoru,
Nochi Hiromi,
Mori Yoki,
Koyama Jiro
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81183-4
Subject(s) - phorbol , guinea pig , nadph oxidase , reductase , phosphorylation , biochemistry , chemistry , flavoprotein , protein kinase c , cytochrome c , microbiology and biotechnology , enzyme , biology , mitochondrion , endocrinology
The plasma membrane‐bound NADPH‐cytochrome c reductase of guinea pig macrophages (Mφ) was found to be phosphorylated when [ 32 P]phosphate‐labeled cells were stimulated with 12‐phorbol 13‐myristate acetate (PMA). The time course of phosphorylation was parallel to that of O − 2 ‐generating activity elicited. These results suggest that the reductase participates as a flavoprotein in activation of the respiratory burst NADPH oxidase, when phosphorylated.