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Stimulation of progesterone and prostaglandin E 2 production by lipoxygenase metabolites of arachidonic acid
Author(s) -
Wang Jian,
Yuen Basil Ho,
Leung Peter C.K.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81182-2
Subject(s) - arachidonic acid , medicine , endocrinology , stimulation , lipoxygenase , prostaglandin e2 , luteinizing hormone , prostaglandin , chemistry , hormone , biology , enzyme , biochemistry
The role of several lipoxygenase metabolites of arachidonic acid in the action of luteinizing homone‐releasing hormone (LHRH) on ovarian hormone production was investigated. Like LHRH, treatment of rat granulosa cells with 5‐HETE, 5‐HPETE, 12‐HETE, 15‐HETE or 15‐HPETE stimulated progesterone (P) and prostaglandin E 2 (PGE 2 ) production. 12‐HEPE was most potent and stimulated P and PGE 2 equally well. By contrast, 5‐HETE stimulated P better than PGE 2 , while 15‐HETE was a potent stimulator of PGE 2 but not of P. Stimulation of P and PGE 2 by LHRH or 12‐ O ‐tetradecanoylphorbol 13‐acetate (TPA) was further augmented by several HETEs and HPETEs. Like protein kinase C, arachidonic acid metabolites appear to mediate the multiple actions of LHRH in the ovary.