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Elevated levels of the guanine nucleotide binding protein, G o , are associated with differentiation of neuroblastoma × glioma hybrid cells
Author(s) -
Mullaney Ian,
Milligan Graeme
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81174-3
Subject(s) - neuroblastoma , guanine , antiserum , cellular differentiation , pertussis toxin , g protein , biology , microbiology and biotechnology , intracellular , glioma , nucleotide , biochemistry , cell culture , gene , antibody , receptor , immunology , cancer research , genetics
Each of a range of pharmacological agents which function to increase intracellular levels of cAMP caused a morphological ‘differentiation’ of neuroblastoma × glioma hybrid, NG108‐15, cells grown in tissue culture. Associated with this differentiation, increased incorporation of [ 32 P]ADP‐ribose catalysed by pertussis toxin was noted into a band of some 39–40 kDa in membranes derived from these cells. Immunoblotting using two antipeptide antisera which identify different regions of G o α demonstrated marked increases in the levels of this polypeptide in membranes of the differentiated cells. However, levels of the β‐subunit did not increase appreciably with differentiation.