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cDNA cloning and nucleotide sequence of lipocortin‐like 33 kDa protein in guinea pig neutrophils
Author(s) -
Sato Eisuke F.,
Tanaka Yoshikazu,
Utsumi Kozo
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81173-1
Subject(s) - complementary dna , microbiology and biotechnology , biology , cdna library , primer (cosmetics) , nucleic acid sequence , peptide sequence , guinea pig , homology (biology) , amino acid , molecular cloning , biochemistry , chemistry , gene , genetics , organic chemistry
cDNA clones of guinea pig neutrophil 33 kDa protein, a lipocortin like‐protein, were isolated from two λgt10 libraries and one primer‐extended λgt10 library of guinea pig neutrophils using synthetic oligonucleotide probes or cDNA fragment probe. The cDNA consists of 1389 nucleotides, and contains 1038 nucleotides encoding 346 amino acids of 33 kDa protein and a poly(A) tail. The deduced amino acid sequence shows high homology with those of lipocortin 1 from human U937 cells (89% homology) and rat lung (86%).