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Enhanced levels of cathepsin B mRNA in murine tumors
Author(s) -
Moin Kamiar,
Rozhin Jurij,
McKernan Timothy B.,
Sanders Virginia J.,
Fong Dunne,
Honn Kenneth V.,
Sloane Bonnie F.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81162-7
Subject(s) - cathepsin b , microbiology and biotechnology , messenger rna , cathepsin d , rna , cathepsin l1 , biology , cathepsin , melanoma , in situ hybridization , cathepsin h , cathepsin l , chemistry , cancer research , enzyme , gene , biochemistry
Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16‐F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two species of cathepsin B specific RNA transcripts (2.2 and 4.1 kb) in total RNA preparation of all four tissues. The concentrations of the 2.2 and 4.1 kb species were 3.6 and 2.7‐fold greater in the highly metastatic B16a melanoma than in normal liver. The concentration of the 2.2 kb species in the invasive hepatoma wa 1.7‐fold greater than in normal liver. The increased levels of the 2.2 kb message were reflected in increases in activity of cathepsin B in both Hepa cl 9 and B16a.