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Molecular cloning and sequencing of human cDNA for phosphoribosyl pyrophosphate synthetase subunit II
Author(s) -
Iizasa Taizo,
Taira Masanori,
Shimada Hideaki,
Ishijima Sumio,
Tatibana Masamiti
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81159-7
Subject(s) - complementary dna , protein subunit , amino acid , biochemistry , nucleotide , pyrophosphate , biology , coding region , enzyme , homology (biology) , cloning (programming) , microbiology and biotechnology , peptide sequence , gene , chemistry , computer science , programming language
cDNA clones for human phosphoribosyl pyrophosphate synthetase subunit II (PRS II) were isolated. The five overlapping clones contained 2457 base pairs (bp) covering a 954‐bp complete coding region for 318 amino acid residues. Homologies between human and rats PRS II were 99% of the amino acid and 88% of the nucleotides in the coding region. This amino acid homology seems to be the highest so far reported for enzymes involved in nucleotide metabolism and glycolysis. The highly conserved structure may be required for unique catalysis and rigid regulation of this enzyme.