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Novel 1 H‐ 31 P heteronuclear coherence transfer spectroscopy based on spin locking MLEV‐17 and its application to signal assignment for a short DNA fragment
Author(s) -
Hiroaki Hidekazu,
Uesugi Seiichi
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81158-5
Subject(s) - heteronuclear molecule , proton , chemistry , spectroscopy , coherence (philosophical gambling strategy) , pulse sequence , oligonucleotide , two dimensional nuclear magnetic resonance spectroscopy , phosphorus , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , nuclear magnetic resonance , atomic physics , dna , physics , stereochemistry , nuclear physics , biochemistry , organic chemistry , quantum mechanics , chromatography
We have applied the MLEV‐17 proton spin locking pulse to 1 H‐ 31 P 2D heteronuclear correlation spectroscopy. Using this technique, long‐range correlations between a proton and phosphorus that is up to 5 bonds distant are observed. Thus, the 3′‐, 4′‐ and 5′‐proton resonances can be traced ‘sequentially’ along the chain on the 2D NMR chart. Application of this technique to the complete assignment of the sugar proton and phosphorus resonances for d(ApGpA) is reported. This technique can also be used for convenient assignment of the phosphorus resonances of oligonucleotides as correlations between the 1′‐proton and its 3′‐side phosphorus are observed.