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Parallel stranded DNA with AT base pairing
Author(s) -
Shchyolkina A.K.,
Lysov Yu.P.,
Il'ichova I.A.,
Chernyi A.A.,
Golova Yu.B.,
Chernov B.K.,
Gottikh B.P.,
Florentiev V.L.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81157-3
Subject(s) - aqueous solution , base pair , oligonucleotide , chemistry , crystallography , dna , spectral line , helix (gastropod) , base (topology) , denaturation (fissile materials) , analytical chemistry (journal) , chromatography , nuclear chemistry , physics , biochemistry , biology , ecology , mathematical analysis , mathematics , astronomy , snail
The concentration and temperature dependences of the UV and CD spectra of the oligonucleotide 3′‐d(ApTpApTpApTpApTpApTp)‐O(CH 2 ) 6 O‐5′‐d(pApTpApTpApTpApTpApT) (eicosamer) in aqueous solution at pH 7 in the presence of 0.5 M NACl were studied. At less than 10 −6 M, the eicosamer was shown to form in solution a hairpin with parallel orientation of chains (parallel hairpin). From thermal denaturation profiles [ A 260 ( T )] the thermodynamic parameters, ΔH°, ΔS° and T m for parallel hairpin formation were calculated to be −90±8 kJ/mol. −300±20 J · mol −1 · K −1 and 40.5°C, respectively. The CD spectra of the parallel double helix differed from those of B‐form DNA and had characteristic features: decreasing magnitude of the positive maximum at 265 nm and a negative peak at 285 nm.