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Isolation and expression in Saccharomyces cerevisiae of a gene encoding an α‐amylase from Schwanniomyces castellii
Author(s) -
Abarca Dolores,
Fernández-Lobato María,
Claros Manuel Gonzalo,
Jiménez Antonio
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81144-5
Subject(s) - saccharomyces cerevisiae , gene , biology , microbiology and biotechnology , genetics , gene expression , saccharomyces , molecular cloning , yeast , cloning (programming) , computer science , programming language
A gene ( SWA1 ) encoding an α‐amylase activity from Schwanniomyces castellii has been cloned and expressed, via yeast cloning vector YEp13, in Saccharomyces cerevisiae . By using a riboprobe which is internal to the SWA1 gene, a 1.55 kb transcript was detected in the poly(A) + RNA from both Sw. castellii and a S. cerevisiae clone harboring the SWA1 gene. This transcript should, therefore, correspond to the SWA1 gene. In addition, the DNA strand determining the α‐amylase activity has been defined. Transcription of the SWA1 gene appears to be highly regulated in Sw. castellii , whereas it is constitutive in the S. cerevisiae harboring this gene.