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Evidence for the involvement of carboxyl groups in passive calcium uptake by liver plasma membrane vesicles and in agonist‐induced calcium uptake by hepatocytes
Author(s) -
Karjalainen Ari,
Bygrave Fyfe L.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81141-x
Subject(s) - vesicle , chemistry , agonist , calcium , membrane , biochemistry , hepatocyte , reagent , biophysics , in vitro , receptor , biology , organic chemistry
The hydrophobic reagents DCCD and EEDQ, each of which reacts with protein carboxyl groups, were found to inhibit both passive Ca 2+ uptake by plasma membrane vesicles isolated from rat liver and agonist‐induced Ca 2+ uptake by hepatocytes. The data raise the possibility that the Ca 2+ inflow pathway(s) in liver has a specific requirement for a reactive carboxyl group or groups.