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Correlation of trenalose content and heat resistance in yeast mutants altered in the RAS/adenylate cyclase pathway: is trehalose a thermoprotectant?
Author(s) -
Hottiger Thomas,
Boller Thomas,
Wiemken Andres
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81139-1
Subject(s) - trehalose , yeast , saccharomyces cerevisiae , adenylate kinase , mutant , heat shock protein , biochemistry , wild type , biology , heat shock , hsp70 , cyclase , chemistry , microbiology and biotechnology , enzyme , gene
Trehalose content and thermotolerance were closely correlated in wild type yeast ( Saccharomyces cerevisiae ) and in cyr1‐2 and bcy1‐1 mutants both during exponential growth at 27°C and during heat shock at 40°C. Trehalose levels were high when heat shock proteins (hsps) were expected to be induced and low when hsps were presumably absent. It was tried to uncouple trehalose biosynthes and hsp‐induction. Various non‐heat stresses affected trehalose levels of wild type cells in a similar way as they would have affected hsps. However, no trehalose was accumulated when cells were treated with canavanine, a well‐known inducer of hsps but not of the thermotolerant state.