Premium
Purification of a DNA methyltransferase from Bacillus natto B3364
Author(s) -
Kim Ella L.,
Maliuta Staniclav S.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81122-6
Subject(s) - methyltransferase , dna methyltransferase , gel electrophoresis , dna , chemistry , methylation , biochemistry , size exclusion chromatography , polyacrylamide gel electrophoresis , cytosine , methionine , molecular mass , microbiology and biotechnology , biology , enzyme , amino acid
An S‐adenosyl‐L‐methionine:DNA‐methyltransferase, termed M · Bna I, was purified from Bacillus natto B3364 strain by successive column chromatography. The molecular weight determined by gel filtration was 37 kDa for M · Bna I. Analysis of methyltransferase by sodium dodecyl sulfate—polyacrylamide gel electrophoresis showed correspondence of the M · Bna I activity with one protein band at a molecular weight of 35 kDa. Sequencing of pUC19 DNA methylated with M · Bna I showed the cytosine‐5 methylation in the Bna I recognition sequence GGAT↓CC at the position indicated by the arrow.