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Expression of the cDNA encoding lipocortin‐like 39 kDa protein of guinea pig neutrophils in yeast
Author(s) -
Sato Eisuke F.,
Tanaka Yoshikazu,
Edashige Keisuke,
Kobuchi Hirotsugu,
Morishita Seiji,
Sugino Yasuo M.,
Inoue Masayasu,
Utsumi Kozo
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81097-x
Subject(s) - complementary dna , guinea pig , yeast , microbiology and biotechnology , biology , annexin , gene , biochemistry , genetics , flow cytometry
The cDNA encoding lipocortin‐like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A 2 , Ca 2+ ‐dependent binding to acidic‐phospholipids and F‐actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.