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Inhibition of protein kinase C induces differentiation of neuroblastoma cells
Author(s) -
Miñana Maria-Dolores,
Felipo Vicente,
Grisolia Santiago
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81087-7
Subject(s) - neurite , protein kinase c , protein kinase a , microbiology and biotechnology , kinase , cellular differentiation , neuroblastoma , chemistry , mitogen activated protein kinase kinase , biology , biochemistry , cell culture , in vitro , gene , genetics
It is shown that 1‐(5‐isoquinolinylsulfonyl)‐2‐methylpiperazine (H7), a specific inhibitor of protein kinase C, induces neuritogenesis in neuro 2a cells. The percentage of differentiated cells was 9%, 20%, 59% and 85% at 0, 17, 85 and 500 μM H7, respectively. The number of neurites/cell increased 2‐, 8‐ and 14‐fold over the controls for 17, 85 and 500 μM H7, respectively. These results indicate that protein kinase C plays a key role in the control of differentiation of neural cells and that its specific inhibition may be of basic as well as of practical importance.