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RNA degrading activity is tightly associated with the multicatalytic proteinase, ingensin
Author(s) -
Tsukahara Toshifumi,
Tanaka Ken-ichi,
Ogawa Toshio,
Ishiura Shoichi,
Funabiki Ryuhei,
Sugita Hideo
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81086-5
Subject(s) - rna , proteinase k , biochemistry , elution , ribosomal rna , chemistry , enzyme , biology , gene , chromatography
The multicatalytic proteinase, ingensin, was purified to homogeneity from chicken liver. rRNA‐degrading activity was co‐eluted with the purified multicatalytic proteinase from a TSK‐3000SW column. This RNA‐degrading activity was inactivated by heat treatment and the addition of a low concentration of SDS. Therefore, the RNA‐degrading activity co‐eluted with the multicatalytic proteinase was not due to contamination by low‐molecular‐mass RNases. These results strongly suggest that this RNA‐degrading activity was tightly associated with the multicatalytic proteinase, ingensin.