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α‐Bungarotoxin interacts with the rat brain tachykinin receptors
Author(s) -
Utkin Yu.N.,
Lazakovich E.M.,
Kasheverov I.E.,
Tsetlin V.I.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81071-3
Subject(s) - tachykinin receptor , receptor , chemistry , neuroscience , biochemistry , biology , substance p , neuropeptide
α‐Bungarotoxin (αBgt) was shown to inhibit the binding of the 125 I‐labeled substance P (SP) and eledoisin (EL) to the rat brain membranes with K 1 values of 8.0±5.0 × 10 −8 and 1.1±0.5 × 10 −6 M, respectively. Lower inhibitory activity was manifested by several other postsynaptically acting snake venom neurotoxins. The αBgt inhibition of SP binding with a K 1 value of 8.5±5.5 × 10 −8 M to solubilized preparations of the rat brain membranes was demonstrated. The capacity to displace SP was found for d‐tubocurarine and phencyclidine, although at concentrations considerably higher than those affecting the nicotinic acetylcholine receptors (AChRs). The results obtained suggest that some of the αBgt‐binding polypeptides, distinct from neuronal AChRs, may be functionally associated with the tachykinin receptors (TchR).