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Primary structures of new ‘iso‐hirudins’
Author(s) -
Scharf M.,
Engels J.,
Tripier D.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81070-1
Subject(s) - hirudin , leech , protein primary structure , primary (astronomy) , high performance liquid chromatography , chemistry , fragmentation (computing) , thrombin , peptide sequence , biochemistry , sequence (biology) , stereochemistry , enzyme , chromatography , gene , biology , immunology , astronomy , ecology , platelet , physics , world wide web , computer science
Crude hirudin (12.7 U/μg), a complex mixture of polypeptides obtained from the leech, could be separated by microbore HPLC. A combination of amino acid analysis, N‐terminal microsequencing and chemical as well as enzymatic fragmentation made the primary sequence of the new isohirudins Ia‐IIIb' accessible. The biological activity determined in the thrombin inhibition test showed a comparable value for all of these compounds. The results presented address the question as to whether these isohirudins are true mutations from a family of genes or a family of leeches.